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    Breaking News

    Saliva Tests Could Spot Silent COVID-19 Carriers

    Mass screening with self-collected saliva and rapid testing could provide easy, non-invasive, quick, relatively accurate results.

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    Hokkaido University09.29.20
    Testing self-collected saliva samples could offer an easy and effective mass testing approach for detecting asymptomatic COVID-19.
     
    Scientists at Hokkaido University and colleagues in Japan have demonstrated a quick and effective mass testing approach using saliva samples to detect individuals who have been infected with COVID-19 but are still not showing symptoms. Their findings were published in the journal Clinical Infectious Diseases.
     
    "Rapid detection of asymptomatic infected individuals will be critical for preventing COVID-19 outbreaks within communities and hospitals," said Hokkaido University researcher Takanori Teshima, who led the study.
     
    Many of the world's governments are showing reluctance to re-institute full national lockdowns as second waves of COVID-19 infections loom on the horizon. Testing and tracing systems will need to be ramped up in order to detect and isolate people who have the virus as early as possible.
     
    Teshima and colleagues tested and compared the nasopharyngeal swabs and saliva samples of almost 2,000 people in Japan who did not have COVID-19 symptoms. Two different virus amplification tests were performed on most of the samples: the PCR test, which is now well-known and widely available around the world, and the less commonly used but faster and more portable RT-LAMP test.


    Both nasopharyngeal swab and saliva testing showed high sensitivity and specificity to the SARS-CoV-2. Image courtesy of Isao Yokota et al., Clinical Infectious Diseases, September 25, 2020.
     
    The number of positive and negative results in all samples was very similar, with the nasopharyngeal swabs and saliva samples able to detect those with the infection in 77-93 percent and 83-97 percent of subjects, respectively. Both two tests were also able to identify those without the infection in greater than 99.9 percent of subjects. The virus loads detected in nasopharyngeal swab and saliva were equivalent and highly correlated. Teshima said, "PCR sensitivity is much higher than previously thought 70 percent that came from initial data of symptomatic patients."
     
    While finding both nasopharyngeal and saliva samples have high sensitivity and specificity to the SARS-CoV-2, Teshima said "Saliva testing has significant logistic advantages over the commonly used nasopharyngeal swab testing." "Self-collection of saliva is painless for examinees, and more importantly, it eliminates the close contact with the examiners, reducing the risk of viral exposure."
     
    "We also found that it is unlikely that the sensitivity of RT-LAMP is significantly less than that of the PCR test, suggesting that it might be a useful alternative for diagnosing COVID-19 infection, especially where diagnosis is required at the point of sample collection, like in sports venues or at airports," said Teshima.
     
    Researchers point to a limitation of the study that they did not follow up with clinical outcomes. Nonetheless, they suggest that the results give good indication that mass screening using self-collected saliva and rapid RT-LAMP testing could provide easy, non-invasive, quick and relatively accurate results, with minimal risk of viral transmission to healthcare workers.
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    CURRENT ISSUE

    April 2021

    • Transporting Treatment: An Examination of Tubing Technologies
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    • View More >

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